Description
The antibody has a proven strong Biotin-Streptavidin/HRP immunostaining at a 1/500-1/1000 dilution in human pancreatic islets. Staining is completely eliminated by pretreatment of the diluted antibody in an excess of glucagon.
Preadsorption of the diluted antibody with an excess of the following substances had no effect on glucagon labeling: secretin, vasoactive intestinal peptide, peptide histidine isoleucine-27, gastric inhibitory polypeptide, rat and human growth hormone releasing hormone and somatostatin.
Photo Description: IHC image of alpha cells in the islets of Langerhans of the rat pancreas staining for glucagon (above) and counterstained with hemotoxylin (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:1000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Host: Rabbit
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Chick, Human, Mouse, Pig, Rat, Sea Lamprey, Snail, Three-Spined Stickleback (Fish), Turtle
Availability: In Stock
Alternate Names: anti-glucagon
Gene Symbol: GCG
RRID: AB_572241
Database Links:
Entrez Gene: 396196 Chick
Entrez Gene: 2641 Human
Entrez Gene: 14526 Mouse
Entrez Gene: 397595 Pig
Entrez Gene: 24952 Rat
Entrez Gene: 102943761 Turtle
