Entries by admin_3fx7e0oy

5-HT (Serotonin) 6 Receptor Antibody

The antibody is provided as 100 uL of affinity purified serum in PBS (0.02 M sodium phosphate with 0.15 M sodium chloride, pH 7.5) with 1% BSA (bovine serum albumin), and 0.02% sodium azide. Reacts with rat species. The ImmunoStar 5HT 6 receptor antibody was quality control tested using standard immunohistochemical methods. The antiserum demonstrates […]

C-FOS Antibody

For induction of c-fos protein activity rats were injected with 1.0 ml of 1.5 M NaCl per 100 grams of body weight. Negative control rats were injected with the same volume of normal saline. The ImmunoStar c-fos antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat paraventricular […]

Neuropeptide Y Y1 Receptor Antibody

The ImmunoStar Neuropeptide Y Y1 Receptor was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex, arcuate and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/500 – 1/1000 in PBS – Bn/Av-HRP detection. The antibody was characterized by immunohistochemistry and Western blot. Western blot […]

5-HT (Serotonin) 2C Receptor Antibody

The ImmunoStar 5-HT2C receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat choroid plexus and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are /500 – 1/1000 in PBS – Bn/Av-HRP technique. Intensification methods such as nickel will approximately double the dilution factor as […]

5-HT (Serotonin) 1A Receptor Antibody

The histochemical antibody for 5-HT1A receptor is generated in a rabbit against synthetic peptide sequence corresponding to amino acids 294-312 of the rat 5-HT1A receptor. The antiserum is provided as 100 µl of affinity purified serum containing 1% BSA. Controls: The ImmunoStar 5-HT1A receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum […]

GAT-2 (Gamma Aminobutyric Acid Transporter) Antibody

The ImmunoStar GAT-2 antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat retina and leptomeninges using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/500 – 1/1,000 in PBS/0.3% Triton X-100 – biotin/avidin-HRP Technique. The antiserum has been characterized as specific to GAT-2; please see references […]

nNOS:N-Terminal Peptide Control

Pre-adsorption of nNOS (N-terminal) antiserum, diluted according to the antibody specification sheet, with 5 µg/ml nNOS (N-terminal) peptide immunogen following the instructions below provides complete blockage of nNOS (N-terminal) immunolabeling. The peptide is provided as 25 µg of lyophilized human nNOS (N-terminal), and 0.09% sodium azide, sequence 134-148. Please read the instructions carefully before beginning […]

5-HTP (5-Hydroxytryptophan) Antibody

The antibody has a proven maximum biotin-streptavidin/HRP staining at a 1/1000 – 1/2000 dilution in rat raphe nuclei. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published […]

Calretinin Antibody

The antibody has a proven maximum biotin-avidin/HRP staining at a 1/1000 – 1/4000 dilution in rat cortex, hippocampus and hypothalamus. The antiserum has been characterized as specific to calretinin; please see references listed below. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended Photo Description: IHC image of […]