The antibody is provided as 100 uL of affinity purified serum in PBS (0.02 M sodium phosphate with 0.15 M sodium chloride, pH 7.5) with 1% BSA (bovine serum albumin), and 0.02% sodium azide. Reacts with rat species.
The ImmunoStar 5HT 6 receptor antibody was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex, amygdala and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/300 – 1/500 in PBS/0.3% Triton X-100 – Bn/Av-HRP Technique . The addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solution will approximately double the dilution factor as recommended.
Immunolabeling is completely abolished by preadsorption with synthetic rat 6 receptor. Immunolabeling of Western blot revealed a single band of approximately 53kD. Due to the difficulty in running westerns with this antibody, ImmunoStar does not support or warrant the western blot application. The western information is included as specificity information only.